5AC3
Crystal structure of PAM12A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-11-17 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.870, 82.870, 112.891 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.770 - 1.800 |
| R-factor | 0.16159 |
| Rwork | 0.160 |
| R-free | 0.19608 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m22 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.295 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.400 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.140 | 0.760 |
| Number of reflections | 42172 | |
| <I/σ(I)> | 12.8 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | VAPOR DIFFUSION, HANGING DROP, 1.0 M SODIUM ACETATE, 0.1 M HEPES PH 7.0, 0.02M CADMIUM SULFATE |
