5ABR
Structure of FeSI protein from Azotobacter vinelandii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-02 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.040, 60.100, 45.510 |
| Unit cell angles | 90.00, 109.37, 90.00 |
Refinement procedure
| Resolution | 42.934 - 2.110 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.23070 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.373 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.930 | 2.160 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.130 | 0.530 |
| Number of reflections | 11386 | |
| <I/σ(I)> | 8.1 | 2.4 |
| Completeness [%] | 98.7 | 97.9 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPES PH 7.5, 77% V/V MPD |
