5ABM
Sheep aldehyde dehydrogenase 1A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.985, 80.804, 171.408 |
| Unit cell angles | 90.00, 117.97, 90.00 |
Refinement procedure
| Resolution | 151.850 - 1.700 |
| R-factor | 0.18126 |
| Rwork | 0.180 |
| R-free | 0.20268 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4x4l |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.810 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.800 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.110 | 0.860 |
| Number of reflections | 249604 | |
| <I/σ(I)> | 12.6 | 2.1 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | 100 MM BIS-TRIS, PH 6.0, 4.5-7% PEG5000, 150=225MM MGCL2, 4DEGREE |
