5AAO
Crystal structure of fluorogen-activating designed ankyrin repeat protein (DARPin) dimer in complex with malachite green
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-25 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.512, 109.754, 100.301 |
| Unit cell angles | 90.00, 94.35, 90.00 |
Refinement procedure
| Resolution | 19.951 - 2.600 |
| R-factor | 0.2159 |
| Rwork | 0.213 |
| R-free | 0.26220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1svx |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.453 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.273 | |
| Number of reflections | 62041 | |
| <I/σ(I)> | 6.6 | 0.86 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 2.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% (W/V) PEG3350, 0.2 M SODIUM MALONATE, PH 5.0 |
