5A95
Crystal structure of beta-glucanase SdGluc5_26A from Saccharophagus degradans in complex with tetrasaccharide A, form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-11 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.048, 60.384, 130.326 |
Unit cell angles | 90.00, 104.75, 90.00 |
Refinement procedure
Resolution | 45.440 - 1.350 |
R-factor | 0.13071 |
Rwork | 0.129 |
R-free | 0.15610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a8n |
RMSD bond length | 0.010 |
RMSD bond angle | 1.427 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.630 | 1.420 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.060 | 0.440 |
Number of reflections | 237302 | |
<I/σ(I)> | 10.5 | 2.6 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 30% (W/V) PEG 4000, 0.1 M TRIS BUFFER PH 7.5, 0.2 M MGCL2 |