5A8J
Crystal structure of the ArnB paralog VWA2 from Sulfolobus acidocaldarius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.640, 74.280, 145.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.658 - 1.460 |
| R-factor | 0.1659 |
| Rwork | 0.165 |
| R-free | 0.20130 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.340 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CCP4 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.660 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.050 | 0.490 |
| Number of reflections | 65094 | |
| <I/σ(I)> | 17.5 | 3.5 |
| Completeness [%] | 99.2 | 97.7 |
| Redundancy | 5.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | RESERVOIR BUFFER: 0.1 M SODIUMCITRATE PH 5.5; 2.5 M AMMONIUMSULFATE; PROTEIN BUFFER: 20 MM HEPES PH 7.0; 100 MM KCL, 2.7 MG/ML PROTEIN IN PROTEIN BUFFER; MIX RESERVOIR AND PROTEIN 1:1 |
