5A8B
Structure of a parallel dimer of the aureochrome 1a LOV domain from Phaeodactylum tricornutum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-19 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.391, 75.550, 77.664 |
Unit cell angles | 90.00, 94.73, 90.00 |
Refinement procedure
Resolution | 38.700 - 2.791 |
R-factor | 0.1733 |
Rwork | 0.170 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ue6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.208 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.700 | 2.970 |
High resolution limit [Å] | 2.790 | 2.790 |
Rmerge | 0.120 | 0.300 |
Number of reflections | 16065 | |
<I/σ(I)> | 8.5 | 308 |
Completeness [%] | 98.9 | 94.3 |
Redundancy | 4.1 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 24%(W/V) PEG1500, 20%(V/V) GLYCEROL, pH 8 |