5A6W
Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikD with the HMA domain of Pikp1 from rice (Oryza sativa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 118.410, 118.410, 35.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.470 - 1.600 |
| R-factor | 0.17908 |
| Rwork | 0.178 |
| R-free | 0.20544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a6p |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.567 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.470 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.650 |
| Number of reflections | 32549 | |
| <I/σ(I)> | 32.3 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.7 | 17.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2M AMMONIUM SULPHATE, 0.1M CHES PH 9 AND 20% PEG335 |
