5A6S
Crystal structure of the CTP1L endolysin reveals how its activity is regulated by a secondary translation product
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 136.197, 136.197, 56.457 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.900 - 1.900 |
R-factor | 0.16412 |
Rwork | 0.162 |
R-free | 0.20468 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1JFX AND 2NW0 HYBRID MODEL |
RMSD bond length | 0.020 |
RMSD bond angle | 1.800 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.150 | 0.750 |
Number of reflections | 40146 | |
<I/σ(I)> | 9.6 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.2 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5-10 % PEG 8000, 20 MM TRIS PH 8.0 |