5A6R
Crystal structure of the BTB domain of human KCTD17
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-12 |
Detector | DECTRIS PILATUS |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 285.743, 285.743, 285.743 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 86.155 - 2.850 |
R-factor | 0.2213 |
Rwork | 0.220 |
R-free | 0.24180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3drz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.552 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.150 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.100 | 1.500 |
Number of reflections | 23906 | |
<I/σ(I)> | 17.1 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.8 | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 18% MPD -- 0.1M TRIS PH 8.5 |