5A4U
AtGSTF2 from Arabidopsis thaliana in complex with indole-3-aldehyde
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2014-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.860, 94.410, 152.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.380 - 2.000 |
| R-factor | 0.19935 |
| Rwork | 0.198 |
| R-free | 0.23353 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnw |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.849 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.410 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.630 |
| Number of reflections | 86285 | |
| <I/σ(I)> | 17.2 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1M PCTP (PROPIONIC ACID, CACODYLATE, BIS-TRIS PROPANE SYSTEM, 15% (W/V) PEG 1.5K PH 7.0. PROTEIN AT 10 MG PER ML |
