5A39
Structure of Rad14 in complex with cisplatin containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 |
| Unit cell lengths | 54.421, 54.421, 130.773 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.420 - 2.800 |
| R-factor | 0.21462 |
| Rwork | 0.213 |
| R-free | 0.25554 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.570 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.400 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.150 | 1.330 |
| Number of reflections | 9402 | |
| <I/σ(I)> | 9.1 | 1.2 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M MAGNESIUM ACETATE 0.1M MES PH 6.5 30% MPD |
