5A2F
Two membrane distal IgSF domains of CD166
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-04 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.320, 72.320, 105.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.090 - 1.860 |
| R-factor | 0.2225 |
| Rwork | 0.222 |
| R-free | 0.23910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cjj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS (VIA XIA2) |
| Data scaling software | Aimless (VIA XIA2) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.140 | 1.910 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.080 | 0.660 |
| Number of reflections | 24110 | |
| <I/σ(I)> | 20.1 | 3.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 13 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M SODIUM HEPES, PH 7.5, 25% PEG-2000 MME |
