5A2A
Crystal Structure of Anoxybacillus Alpha-amylase Provides Insights into a New Glycosyl Hydrolase Subclass
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Collection date | 2014-07-10 |
Detector | RIGAKU R-AXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.429, 63.443, 122.758 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 1.900 |
R-factor | 0.1799 |
Rwork | 0.177 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e2o |
RMSD bond length | 0.023 |
RMSD bond angle | 2.232 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.420 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.060 | 0.370 |
Number of reflections | 34971 | |
<I/σ(I)> | 13.3 | 2.5 |
Completeness [%] | 98.1 | 93.4 |
Redundancy | 2.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.2 M CALCIUM ACETATE, 0.1 M SODIUM CACODYLATE (PH 6.5) 18% (W/V) POLYETHYLENE GLYCOL 8, 000 |