5A1K
Crystal structure of calcium-free human adseverin domains A1-A3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.410, 65.250, 105.190 |
| Unit cell angles | 90.00, 118.27, 90.00 |
Refinement procedure
| Resolution | 59.630 - 2.900 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1D0N RESIDUES 27-370 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.600 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.150 | 1.330 |
| Number of reflections | 17766 | |
| <I/σ(I)> | 10.4 | 1.5 |
| Completeness [%] | 98.0 | 95 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20 % POLYETHYLENE GLYCOL 3350, 100 MM BIS-TRIS PROPANE PH 7.5 |
