5A1I
The structure of Human MAT2A in complex with SAM, Adenosine, Methionine and PPNP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 67.972, 94.074, 117.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.370 - 1.090 |
| R-factor | 0.10481 |
| Rwork | 0.104 |
| R-free | 0.12398 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p02 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.797 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.370 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.090 | 0.090 |
| Number of reflections | 176512 | |
| <I/σ(I)> | 24.5 | 3.4 |
| Completeness [%] | 99.1 | 99 |
| Redundancy | 12.3 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 30 % PEG 600 |
