5A07
X-ray structure of the mannosyltransferase Ktr4p from S. cerevisiae in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-18 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.215, 102.621, 162.651 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.790 - 1.900 |
| R-factor | 0.15798 |
| Rwork | 0.156 |
| R-free | 0.19132 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a08 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.828 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.870 |
| Number of reflections | 81509 | |
| <I/σ(I)> | 15.5 | 2.3 |
| Completeness [%] | 99.1 | 98.9 |
| Redundancy | 9.8 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M NA-CACODYLATE, PH 6.5 0.2M CAOAC 18% (W/V) PEG8000 |
