4ZZV
Geotrichum candidum Cel7A apo structure at 1.4A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-07 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.420, 80.730, 54.910 |
Unit cell angles | 90.00, 109.54, 90.00 |
Refinement procedure
Resolution | 51.750 - 1.370 |
R-factor | 0.17867 |
Rwork | 0.177 |
R-free | 0.20073 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gpi |
RMSD bond length | 0.005 |
RMSD bond angle | 1.066 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.740 | 1.390 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmerge | 0.080 | 0.370 |
Number of reflections | 68773 | |
<I/σ(I)> | 7.3 | 2.6 |
Completeness [%] | 93.9 | 92.4 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.9 | 0.2M MAGNESIUM CHLORIDE HEXAHYDRATE, 20% PEG 3350, pH 5.9 |