4ZZQ
Dictyostelium discoideum cellobiohydrolase Cel7A apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-06 |
| Detector | MARRESEARCH MAR165 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.227, 62.894, 132.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 132.900 - 2.100 |
| R-factor | 0.26067 |
| Rwork | 0.256 |
| R-free | 0.35128 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d5j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.266 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.700 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.330 | |
| Number of reflections | 22434 | |
| <I/σ(I)> | 5.5 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.3 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 12.5% W/V PEG 1000, 12.5% W/V PEG 3350, 12.5% V/V MPD, 0.1 M SODIUM HEPES/MOPS, PH 7.5, 0.03 M MGCL2, 0.03M CACL2 |
