4ZZP
Dictyostelium purpureum cellobiohydrolase Cel7A apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.643, 85.185, 168.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.250 - 2.700 |
| R-factor | 0.23853 |
| Rwork | 0.235 |
| R-free | 0.31337 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d5j |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.269 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.250 | 0.500 |
| Number of reflections | 22787 | |
| <I/σ(I)> | 5.3 | 3.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M SODIUM MALONATE PH 7.0, 12% W/V PEG 3350. |
