4ZZE
Raffinose and panose binding protein from Bifidobacterium animalis subsp. lactis Bl-04, bound with panose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97734 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.880, 91.330, 142.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.113 - 1.760 |
R-factor | 0.1602 |
Rwork | 0.158 |
R-free | 0.20490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zza |
RMSD bond length | 0.005 |
RMSD bond angle | 0.959 |
Data reduction software | xia2 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 23.200 | 1.810 | |
High resolution limit [Å] | 1.760 | 7.870 | 1.760 |
Rmerge | 0.082 | 0.026 | 0.661 |
Rmeas | 0.098 | 0.031 | 0.794 |
Total number of observations | 241213 | ||
Number of reflections | 71009 | 821 | 5173 |
<I/σ(I)> | 10.64 | 28.13 | 2.05 |
Completeness [%] | 99.1 | 87.9 | 99.3 |
Redundancy | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 278 | 0.1 M Tris pH 8.5, 25% PEG 4000, 0.8M magnesium chloride |