4ZZC

The GLIC pentameric Ligand-Gated Ion Channel open form complexed to xenon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.182
Spacegroup name	C 1 2 1
Unit cell lengths	180.000, 132.820, 159.150
Unit cell angles	90.00, 101.88, 90.00

Refinement procedure

Resolution	25.000 - 3.100
R-factor	0.2089
Rwork	0.208
R-free	0.22750
RMSD bond length	0.009
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.270
High resolution limit [Å]	3.100	3.100
Rmerge		0.665
<I/σ(I)>		2
Completeness [%]		99.9
Redundancy		3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	12-15% PEG4000 0.1M Na Acetate pH4 0.4M NaSCN 15% glycerol 5% DMSO