4ZZ7
Crystal structure of methylmalonate-semialdehyde dehydrogenase (DddC) from Oceanimonas doudoroffii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-12-05 |
| Detector | FUJI |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 156.700, 160.300, 238.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.900 |
| R-factor | 0.18802 |
| Rwork | 0.185 |
| R-free | 0.24597 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.440 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.094 | 0.407 |
| Number of reflections | 126662 | |
| <I/σ(I)> | 57.4 | 9.1 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 5.9 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 21% PEG 3350, 0.2 M potassium sodium tartrate pH 7.5 |
