4ZYL

Crystal structure of response regulator RPA3017 in red light signaling of R. palustris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-17
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 65
Unit cell lengths	60.980, 60.980, 200.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.931 - 1.800
R-factor	0.1904
Rwork	0.189
R-free	0.22800
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.308
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	21308
<I/σ(I)>	47
Completeness [%]	99.3
Redundancy	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M sodium citrate tribasic, 0.1M sodium cacodylate pH6.5 and 4% (v/v) 2-propanol