4ZY9
X-ray crystal structure of selenomethionine-labelled V110M mutant of chitosan-binding module 1 derived from chitosanase/glucanase from Paenibacillus sp. IK-5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2014-11-02 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97865 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.233, 65.329, 69.887 |
Unit cell angles | 90.00, 97.21, 90.00 |
Refinement procedure
Resolution | 47.550 - 1.200 |
R-factor | 0.20934 |
Rwork | 0.208 |
R-free | 0.24921 |
Structure solution method | SAD |
RMSD bond length | 0.030 |
RMSD bond angle | 2.466 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.222 | |
Number of reflections | 78378 | |
<I/σ(I)> | 28.4 | 4.5 |
Completeness [%] | 99.8 | 97 |
Redundancy | 6.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium fluoride, PEG 3350 |