4ZY8
K. lactis Lst4 longin domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-12 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 120.703, 120.703, 93.285 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.290 - 2.140 |
R-factor | 0.2126 |
Rwork | 0.211 |
R-free | 0.25220 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.290 | 2.216 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.066 | 0.632 |
Number of reflections | 38603 | |
<I/σ(I)> | 20.4 | 4.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.1 | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 292 | Sodium chloride, Butan-1-ol, Bis-Tris |