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4ZY8

K. lactis Lst4 longin domain

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I02
Synchrotron siteDiamond
BeamlineI02
Temperature [K]100
Detector technologyPIXEL
Collection date2014-08-12
DetectorPSI PILATUS 6M
Wavelength(s)0.979
Spacegroup nameP 42 21 2
Unit cell lengths120.703, 120.703, 93.285
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution35.290 - 2.140
R-factor0.2126
Rwork0.211
R-free0.25220
Structure solution methodSAD
RMSD bond length0.013
RMSD bond angle1.300
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwarePHENIX
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]35.2902.216
High resolution limit [Å]2.1402.140
Rmerge0.0660.632
Number of reflections38603
<I/σ(I)>20.44.4
Completeness [%]100.0100
Redundancy12.111.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.8292Sodium chloride, Butan-1-ol, Bis-Tris

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