4ZXQ
P22 Tail Needle Gp26 1-140 crystallized at pH 3.9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2008-05-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.91 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.952, 94.095, 97.619 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.862 - 2.750 |
R-factor | 0.2167 |
Rwork | 0.215 |
R-free | 0.25180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2poh |
RMSD bond length | 0.004 |
RMSD bond angle | 0.931 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 29142 | |
<I/σ(I)> | 20.4 | 4.3 |
Completeness [%] | 98.1 | 97.9 |
Redundancy | 2.9 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.9 | 293 | 20% PEG 8000, 0.05 M Potassium phosphate monobasic pH 3.9 |