4ZXP

Crystal structure of Peptidyl- tRNA Hydrolase from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-01-23
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	44.718, 73.628, 124.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.550 - 1.630
R-factor	0.173
Rwork	0.171
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p7b
RMSD bond length	0.011
RMSD bond angle	1.266
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CCP4MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.340	1.690
High resolution limit [Å]	1.627	1.630
Number of reflections	49768
<I/σ(I)>	32.3
Completeness [%]	95.0
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	296	0.1M SODIUM CITRATE BUFFER PH 8.0, 0.2M AMMONIUM ACETATE, 25% POLYETHYLENE GLYCOL 4000