4ZXE
X-ray crystal structure of chitosan-binding module 1 derived from chitosanase/glucanase from Paenibacillus sp. IK-5.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-05-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.054, 45.591, 57.581 |
| Unit cell angles | 111.69, 105.36, 97.92 |
Refinement procedure
| Resolution | 50.260 - 1.400 |
| R-factor | 0.14464 |
| Rwork | 0.143 |
| R-free | 0.17179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.414 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.260 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.069 | |
| Number of reflections | 69228 | |
| <I/σ(I)> | 1.8 | 34.2 |
| Completeness [%] | 95.1 | 91.6 |
| Redundancy | 7.9 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Ammonium sulfate, Magnesium chloride, MES, PEG8000 |
