4ZXE

X-ray crystal structure of chitosan-binding module 1 derived from chitosanase/glucanase from Paenibacillus sp. IK-5.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2012-05-21
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	42.054, 45.591, 57.581
Unit cell angles	111.69, 105.36, 97.92

Refinement procedure

Resolution	50.260 - 1.400
R-factor	0.14464
Rwork	0.143
R-free	0.17179
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.027
RMSD bond angle	2.414
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.260	1.420
High resolution limit [Å]	1.400	1.400
Rmerge		0.069
Number of reflections	69228
<I/σ(I)>	1.8	34.2
Completeness [%]	95.1	91.6
Redundancy	7.9	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Ammonium sulfate, Magnesium chloride, MES, PEG8000