4ZWT

Crystal Structure of the Bacteriophage T4 recombination mediator protein UvsY, Lattice Type IV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	184.380, 184.380, 615.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.617 - 4.200
R-factor	0.2754
Rwork	0.274
R-free	0.30780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zwr
RMSD bond length	0.008
RMSD bond angle	1.573
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		4.120
High resolution limit [Å]	4.010	17.940	4.010
Rmerge	0.201	0.058	0.976
Rmeas	0.219	0.064	1.113
Total number of observations	303824
Number of reflections	25618	920	105
<I/σ(I)>	7.35	23.11	1.92
Completeness [%]	56.0	94.5	1.7
Redundancy	6.4		4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	1:1 UvsY (22.5 mg/mL in 0.5 M sodium chloride, 15 mM HEPES, pH 8.2, 2 mM DTT) to 2 M sodium/potassium phosphate, pH 7.0, 0.1 M sodium acetate, pH 4.5