4ZWS
Crystal Structure of the Bacteriophage T4 recombination mediator protein UvsY, Lattice Type III
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 123.571, 123.571, 332.465 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.262 - 2.600 |
R-factor | 0.2037 |
Rwork | 0.202 |
R-free | 0.23630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zwr |
RMSD bond length | 0.007 |
RMSD bond angle | 0.973 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.262 | 2.760 | |
High resolution limit [Å] | 2.600 | 7.710 | 2.600 |
Rmerge | 0.104 | 0.023 | 1.795 |
Rmeas | 0.118 | 0.026 | 2.035 |
Total number of observations | 397409 | ||
Number of reflections | 87352 | 3319 | 14076 |
<I/σ(I)> | 11.26 | 46.65 | 0.91 |
Completeness [%] | 99.9 | 99.3 | 99.7 |
Redundancy | 4.55 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 1:1 UvsY (10 mg/mL, 150 mM sodium chloride, 20 mM HEPES, pH 7.5, 2 mM DTT) to 10% Jeffamine T-403, 10% Jeffamine ED-2003, 100 mM Tris-HCl, pH 8.0 in the presence of 750 uM dA3 oligonucleotide |