4ZWS

Crystal Structure of the Bacteriophage T4 recombination mediator protein UvsY, Lattice Type III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	123.571, 123.571, 332.465
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.262 - 2.600
R-factor	0.2037
Rwork	0.202
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zwr
RMSD bond length	0.007
RMSD bond angle	0.973
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.262		2.760
High resolution limit [Å]	2.600	7.710	2.600
Rmerge	0.104	0.023	1.795
Rmeas	0.118	0.026	2.035
Total number of observations	397409
Number of reflections	87352	3319	14076
<I/σ(I)>	11.26	46.65	0.91
Completeness [%]	99.9	99.3	99.7
Redundancy	4.55		3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	1:1 UvsY (10 mg/mL, 150 mM sodium chloride, 20 mM HEPES, pH 7.5, 2 mM DTT) to 10% Jeffamine T-403, 10% Jeffamine ED-2003, 100 mM Tris-HCl, pH 8.0 in the presence of 750 uM dA3 oligonucleotide