4ZWR

Crystal Structure of the Bacteriophage T4 recombination mediator protein UvsY, Lattice Type II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97930
Spacegroup name	C 2 2 21
Unit cell lengths	85.600, 123.830, 302.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.911 - 3.386
R-factor	0.2405
Rwork	0.239
R-free	0.26650
Structure solution method	SAD
RMSD bond length	0.001
RMSD bond angle	0.333
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	3.520
High resolution limit [Å]	3.386	7.220	3.386
Rmerge	0.151	0.110	0.938
Rmeas	0.163	0.120
Rpim	0.063	0.046	0.393
Total number of observations	93454
Number of reflections	14057
<I/σ(I)>	6.9		1.76
Completeness [%]	63.9	99.6	7.4
Redundancy	6.6	6.6	6.6
CC(1/2)		0.993	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	277	1:1 Se-Met-labeled UvsY (12 mg/mL in 0.5 M sodium chloride, 15 mM HEPES, pH 8.2, 2 mM DTT) to 18% MPD, 225 mM sodium chloride, 0.1 M MES, pH 6.4, sample subjected to reductive alkylation with formaldehyde prior to crystallization