4ZWQ

Crystal Structure of the Bacteriophage T4 recombination mediator protein UvsY, Lattice Type I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	109.260, 109.220, 270.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.727 - 2.351
R-factor	0.2232
Rwork	0.222
R-free	0.25420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zwr
RMSD bond length	0.007
RMSD bond angle	1.111
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.370
High resolution limit [Å]	2.310	10.320	2.310
Rmerge	0.102	0.036	0.224
Rmeas	0.109	0.039	0.252
Total number of observations	352962
Number of reflections	43526	879	131
<I/σ(I)>	13.36	35.55	7.46
Completeness [%]	60.8	96.6	2.5
Redundancy	8.1		5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1:1 UvsY (12.5 mg/mL in 0.5 M sodium chloride, 15 mM HEPES, pH 8.2, 2 mM DTT) to 1.8 M ammonium sulfate, 0.1 M HEPES, pH 7.5, 2% 1,6-hexanediol, sample subjected to reductive alkylation with formaldehyde prior to crystallization