4ZWQ
Crystal Structure of the Bacteriophage T4 recombination mediator protein UvsY, Lattice Type I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 109.260, 109.220, 270.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.727 - 2.351 |
R-factor | 0.2232 |
Rwork | 0.222 |
R-free | 0.25420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zwr |
RMSD bond length | 0.007 |
RMSD bond angle | 1.111 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.370 | |
High resolution limit [Å] | 2.310 | 10.320 | 2.310 |
Rmerge | 0.102 | 0.036 | 0.224 |
Rmeas | 0.109 | 0.039 | 0.252 |
Total number of observations | 352962 | ||
Number of reflections | 43526 | 879 | 131 |
<I/σ(I)> | 13.36 | 35.55 | 7.46 |
Completeness [%] | 60.8 | 96.6 | 2.5 |
Redundancy | 8.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1:1 UvsY (12.5 mg/mL in 0.5 M sodium chloride, 15 mM HEPES, pH 8.2, 2 mM DTT) to 1.8 M ammonium sulfate, 0.1 M HEPES, pH 7.5, 2% 1,6-hexanediol, sample subjected to reductive alkylation with formaldehyde prior to crystallization |