4ZW2
Crystal structure of the Mouse voltage gated calcium channel beta subunit isoform 1a in complex with Alpha Interaction Domain peptide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-06-25 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.240, 69.371, 131.104 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.810 - 1.860 |
R-factor | 0.17054 |
Rwork | 0.169 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t0j |
RMSD bond length | 0.019 |
RMSD bond angle | 1.897 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.053 | 0.364 |
Number of reflections | 36235 | |
<I/σ(I)> | 35.45 | 4.95 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 6.9 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 3350, 0.2 M sodium acetate, 0.1 M bis-tris propane pH 8.0 |