4ZVG
Crystal structure of GGDEF domain of the E. coli DosC - form III
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97627 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.780, 58.930, 113.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.610 - 2.200 |
R-factor | 0.2098 |
Rwork | 0.208 |
R-free | 0.25170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zve |
RMSD bond length | 0.008 |
RMSD bond angle | 1.132 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.099 | 0.783 |
Number of reflections | 18580 | |
<I/σ(I)> | 11.36 | 2.54 |
Completeness [%] | 97.9 | 95.3 |
Redundancy | 5.8 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.2M potassium sodium tartrate, 22% (w/v) PEG 3350 |