4ZVD

Crystal structure of MID domain of the E. coli DosC - form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	P 21 21 21
Unit cell lengths	41.930, 51.260, 92.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.325 - 1.900
R-factor	0.2391
Rwork	0.237
R-free	0.28270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zvc
RMSD bond length	0.004
RMSD bond angle	0.723
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.062	0.624
Number of reflections	16326
<I/σ(I)>	25.22	4.48
Completeness [%]	100.0	100
Redundancy	12.5	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2M ammonium dihydrogen phosphate, 20% (w/v) PEG 3350