4ZVB
Crystal structure of globin domain of the E. coli DosC - form II (ferrous)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.7345 |
Spacegroup name | P 1 |
Unit cell lengths | 47.960, 58.270, 59.400 |
Unit cell angles | 79.52, 75.69, 75.29 |
Refinement procedure
Resolution | 45.341 - 2.400 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.25050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zva |
RMSD bond length | 0.002 |
RMSD bond angle | 0.698 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.130 | 0.752 |
Number of reflections | 22142 | |
<I/σ(I)> | 12.36 | 2.64 |
Completeness [%] | 94.6 | 88.3 |
Redundancy | 10.2 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M sodium phosphate-citrate pH 4.2, 0.2 M sodium chloride, 16% (w/v) PEG 3000 |