4ZVA
Crystal structure of globin domain of the E. coli DosC - form I (ferric)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97627 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 97.410, 97.410, 152.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.705 - 2.000 |
| R-factor | 0.2238 |
| Rwork | 0.222 |
| R-free | 0.25740 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.856 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.684 |
| Number of reflections | 29494 | |
| <I/σ(I)> | 26.44 | 3.52 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 10.7 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M tri-sodium citrate pH 5.6, 0.2 M ammonium acetate, 27% (w/v) PEG 4000 |
