4ZV9
2.00 Angstrom resolution crystal structure of an uncharacterized protein from Escherichia coli O157:H7 str. Sakai
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.615, 96.341, 129.617 |
Unit cell angles | 90.00, 98.22, 90.00 |
Refinement procedure
Resolution | 38.790 - 2.000 |
R-factor | 0.169 |
Rwork | 0.168 |
R-free | 0.19900 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.362 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.052 | 0.550 |
Number of reflections | 115793 | |
<I/σ(I)> | 21.7 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 295 | Crystallization: The JCSG+ Suite (D12): 0.04 M Potasium phosphate, 16% (w/v) PEG 8000, 20% (v/v) glycerol Protein: 8.7 mg/ml in 10 mM Tris-HCl pH 8.3, 0.25 M NaCl, 5 mM BME Freezing: crystallization condition |