4ZV7
Crystal structure of hexagonal form of lipase B from Candida antarctica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-29 |
| Detector | OXFORD TITAN CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 89.034, 89.034, 137.257 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.110 - 2.000 |
| R-factor | 0.14654 |
| Rwork | 0.144 |
| R-free | 0.19276 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w9b |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.964 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 77.110 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 22034 |
| <I/σ(I)> | 24.58 |
| Completeness [%] | 98.1 |
| Redundancy | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294.15 | 24% polyethylene glycol 3350, 0.1M citric acid, and 0.1M sodium acetate |
