4ZV5
Crystal structure of N-myristoylated mouse mammary tumor virus matrix protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 61.927, 61.927, 90.054 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.080 - 1.570 |
| R-factor | 0.2275 |
| Rwork | 0.226 |
| R-free | 0.26270 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.575 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.080 | 1.611 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.039 | |
| Number of reflections | 28486 | |
| <I/σ(I)> | 23.9 | 2.4 |
| Completeness [%] | 99.7 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.2M potassium chloride, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
