4ZV4

Structure of Tse6 in complex with EF-Tu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 41 21 2
Unit cell lengths	176.109, 176.109, 86.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.844 - 3.504
R-factor	0.2326
Rwork	0.230
R-free	0.28340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1efc
RMSD bond length	0.003
RMSD bond angle	0.707
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.500
Number of reflections	32569
<I/σ(I)>	39.4
Completeness [%]	100.0
Redundancy	27.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	22% (w/v) PEG 3350, 0.2M ammonium formate pH 6.6