4ZV3

Crystal structure of the N- and C-terminal domains of mouse acyl-CoA thioesterase 7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	66.847, 106.924, 79.864
Unit cell angles	90.00, 112.32, 90.00

Refinement procedure

Resolution	32.100 - 3.100
R-factor	0.24408
Rwork	0.241
R-free	0.29609
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q2b 2v1o
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.910
High resolution limit [Å]	3.100
Rmerge	0.157
Number of reflections	18941
<I/σ(I)>	5.5
Completeness [%]	99.9
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	200 mM sodium citrate, 12% PEG 3350