4ZV2
An ancestral arginine-binding protein bound to glutamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9655 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.200, 61.300, 104.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.210 - 1.430 |
R-factor | 0.18293 |
Rwork | 0.182 |
R-free | 0.20487 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zv1 |
RMSD bond length | 0.025 |
RMSD bond angle | 2.299 |
Data reduction software | MOSFLM (7.0.9) |
Data scaling software | Aimless (0.1.29) |
Phasing software | MOLREP (11.1.00) |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.200 | 1.450 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.178 | 1.871 |
Number of reflections | 43838 | |
<I/σ(I)> | 9.2 | 1.5 |
Completeness [%] | 99.7 | 96.7 |
Redundancy | 12.7 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.2 M MgCl2, 0.1 M HEPES pH 7.5, 24% (w/v) PEG 3350, microseeds from crushed crystals produced using same precipitant |