4ZUV
Crystal structure of Equine MHC I(Eqca-N*00602) in complexed with equine infectious anaemia virus (EIAV) derived peptide Env-RW12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-05 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.518, 71.441, 99.874 |
| Unit cell angles | 90.00, 102.94, 90.00 |
Refinement procedure
| Resolution | 31.836 - 2.300 |
| R-factor | 0.2013 |
| Rwork | 0.200 |
| R-free | 0.23350 |
| Structure solution method | MIR |
| Starting model (for MR) | 1q94 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.870 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-2000 |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.120 | 0.464 |
| Number of reflections | 50912 | |
| <I/σ(I)> | 12.988 | 3.876 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.02 M magnesium chloride hexahydrare, 0.1 M HEPES, 22% w/v polyacrylic acid sodium salt 5,100 |
