4ZUT

Crystal structure of Equine MHC I(Eqca-N*00602) in complexed with equine infectious anaemia virus (EIAV) derived peptide Gag-GW12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97900
Spacegroup name	P 41 21 2
Unit cell lengths	69.793, 69.793, 207.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.623 - 2.600
R-factor	0.2235
Rwork	0.221
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q94
RMSD bond length	0.005
RMSD bond angle	0.759
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.600	2.500
Rmerge	0.084	0.587
Number of reflections	16706
<I/σ(I)>	38.407	5.314
Completeness [%]	99.2	99.8
Redundancy	11.3	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.02 M magnesium chloride hexahydrare, 0.1 M HEPES, 22% w/v polyacrylic acid sodium salt 5,100