4ZUJ
Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91H at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2015-04-21 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.089, 60.464, 38.262 |
Unit cell angles | 90.00, 93.64, 90.00 |
Refinement procedure
Resolution | 31.030 - 1.720 |
R-factor | 0.1748 |
Rwork | 0.173 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.996 |
Data reduction software | XPREP |
Data scaling software | SAINT |
Phasing software | PHASER (2.5.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.740 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.028 | 0.176 |
Total number of observations | 58673 | |
Number of reflections | 15097 | |
<I/σ(I)> | 22.26 | 4.88 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 3.81 | 2.57 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |