4ZUA

Crystal structure of the ExsA regulatory domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 43 21 2
Unit cell lengths	69.949, 69.949, 191.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.000 - 2.500
R-factor	0.2406
Rwork	0.239
R-free	0.26750
RMSD bond length	0.010
RMSD bond angle	1.130
Data reduction software	MOSFLM
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.210
High resolution limit [Å]	2.500
Rmerge	0.860
Number of reflections	17161
<I/σ(I)>	91
Completeness [%]	99.0
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.6M MgSO4, 0.1M MES pH 6.5 and 0.1M EGTA