4ZU9
Crystal structure of bacterial selenocysteine-specific elongation factor EF-Sec
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-07-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 114.240, 114.240, 204.632 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.390 - 3.191 |
| R-factor | 0.2091 |
| Rwork | 0.206 |
| R-free | 0.26080 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.390 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.380 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.158 | 0.026 |
| Number of reflections | 42987 | |
| <I/σ(I)> | 16.87 | 1.26 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 15.48 | 15.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 293 | 100 mM sodium acetate-HCl buffer, 1.7 M (NH4)2SO4, 20 mM L-cysteine, 3 mM GMPPNP, 10 mM Mg2SO4 |
