4ZU2
Pseudomonas aeruginosa AtuE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8123 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.440, 133.970, 83.350 |
| Unit cell angles | 90.00, 139.46, 90.00 |
Refinement procedure
| Resolution | 19.693 - 2.150 |
| R-factor | 0.2006 |
| Rwork | 0.198 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3H81 and 3I47 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.523 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.250 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.114 | 0.800 |
| Number of reflections | 42012 | |
| <I/σ(I)> | 8.4 | 1.2 |
| Completeness [%] | 95.8 | 90.6 |
| Redundancy | 2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M sodium iodide, 20 % (v/v) PEG 4000 |
